The hygropreference of adult Cryptopygusantarcticus and Alaskozetesantarcticus was investigated over 2 h at 5, 10 and 20°C, along humidity gradients (9–98% RH) established by means of different salt solutions. Two chamber arrangements were employed, linear and grid, to determine any influence of thigmotactic behaviour on distribution within the RH gradient. The humidity preference of both species varied with temperature. At 5 and 10°C, C. antarcticus distributed homogeneously showing no clear RH preference. At 20°C, this species preferred the highest humidity (98% RH). A. antarcticus demonstrated a preference for the lowest humidity (9% RH) at 5°C, but at 10°C its distribution differed between the two arena types. At 20°C, A. antarcticus showed no clear humidity preference. Assays to control for experimental asymmetries along the gradient; thigmotactic behaviour; and aggregative behaviour exclude these factors as explanations for the observed results. The mean initial water content of samples did not differ significantly between temperature regimes (C. antarcticus: 68.6, 71.1 and 74.3%; A. antarcticus: 68.1, 70.1 and 68.6% at 5, 10 and 20°C respectively), but the level of water loss increased significantly with temperature. The influence of desiccation tolerance and the ecological significance of the observed humidity preferences are discussed.